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The pGEM ®-T and pGEM -T Easy Vectors contain all of the features of the pGEM ® -5Zf(+) Vector but are specifically engineered to facilitate the cloning of PCR products.

Technical Manual pGEM®-T and pGEM®-T Easy Vector Systems INSTRUCTIONS FOR USE OF PRODUCTS A1360, A1380, A3600 AND A3610. PRINTED IN USA. Revised 6/09 Part# TM042. 1. Introduction 1.A. Vector Features T-Overhangs for Easy PCR Cloning: The pGEM ®-T and pGEM -T Easy Vectors(a,b) are linearized vectors with a single 3´-terminal thymidine at both ends. The T-overhangs at the insertion.

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Rapid Ligation for the pGEM-T and pGEM-T Easy Vector Systems Citations Roles of 14-3-3β and γ in regulation of the glucocorticoid receptor transcriptional activation and hepatic gluconeogenesis.

For more information, see the pGEM®-T and pGEM®-T Easy Technical Manual #TM042. Recovering a control insert of the correct size indicates the pGEM®-T or pGEM®-T Easy Vector System reagents are performing as they should.

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INSTRUCTIONS FOR USE OF PRODUCTS A1360, A1380, A3600 AND A3610. Briefly centrifuge the pGEM®-T or pGEM®-T Easy Vector and Control Insert.

Rapid Ligation for the pGEM-T and pGEM-T Easy Vector Systems サイテーション Roles of 14-3-3β and γ in regulation of the glucocorticoid receptor transcriptional activation and hepatic gluconeogenesis.

The pGEM®(a,b)-T and pGEM®-T Easy Vector Systems are convenient systems for the cloning of PCR (c) products.The vectors are prepared by cutting Promega's pGEM ® -5Zf(+) (b) and pGEM ® -T Easy Vectors with EcoR V and adding a 3´ terminal.

  1. The pGEM®-T and pGEM®-T Easy Vector Systems include a 2X Rapid Ligation Buffer for ligation of PCR products. Reactions using this buffer may be incubated for 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation.

  2. Technical Manual pGEM®-T and pGEM®-T Easy Vector Systems INSTRUCTIONS FOR USE OF PRODUCTS A1360, A1380, A3600 AND A3610. PRINTED IN USA. Revised 12/10 Part# TM042.

  3. E-mail Promega Technical Services if you have questions on use of this system: Protocol for Ligations Using the pGEM®-T and pGEM®-T Easy Vectors and the 2X Rapid C. Sequence and Multi-Cloning Site of the pGEM®-T Easy Vector.

Cloning Quick PROTOCOL.

The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual.

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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA. Toll Free The pGEM®-T Easy Vector multiple cloning region is flanked.